Fish Haematological Techniques: Haemoglobin Determination

Unit 3 - Haematological Techniques

Haemoglobin Determination	BACK 3 of 3

Place approximately 2ml of the resulting solution into a cuvette, and read the absorbance values in a spectrophotometer at 540nm. The haemoglobin concentration of the blood sample can be calculated from a curve prepared from known standards.
*Definitions*
SPECTROPHOTOMETER: A device that measures reflectance of visible light, or absorbance, as a function of wavelength, thus allowing analysis of colour or comparison of luminous intensities. When light is passed through a solution of a known constituent in a spectrophotometer it measures the absorbance of the solution. This may then be compared to the absorbance of that constituent in a solution of known concentration, thus allowing the concentration of the solution to be determined.