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There is a great deal of variation between laboratories in the procedures employed to carry out blood cell counts.  Shaws counting solutions may be used to estimate leucocyte occurrence (making a 1:200 dilution of blood). 

Blaxhall and Daisley’s method outlined here for  white blood cell counts is widely used.  The same method may be used to carry out erythrocyte counts, although the procedure employed for counting and calculating occurrence of the cells is different.

Make a 1:50 dilution of blood in Dacies fluid.

Definitions

DACIES FLUID:

Formaldehyde:                       10 ml

Trisodium citrate:                 31.3g

Brilliant cresyl Blue:            1.0g

Distilled water                       1 litre